质粒pcDNA-dCas9-p300

质粒编号：61357

Backbone

Vector backbonepc：DNA3.1

Backbone manufacturer：Invitrogen； Life Technologies

Total vector size (bp)：11475

Vector type：Mammalian Expression, CRISPR, Synthetic Biology

Selectable markers：Neomycin (select with G418)

Growth in Bacteria

Bacterial Resistance(s)：Ampicillin, 100 μg/mL

Growth Temperature：30℃

Growth Strain(s)：Stbl3

Growth instructions：May require longer growth times.

Copy number：Low Copy

Gene/Insert

Gene/Insert name：S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300)

Species：H. sapiens (human), Synthetic； S. pyogenes

Insert Size (bp)：6138

Mutation：D10A； H840A in S.pyogenes Cas9

GenBank ID：WP_010922251.1 NP_001420.2

Entrez Gene：EP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)

Promoter：CMV

Tags/ Fusion Proteins：

Flag (N terminal on insert)

SV40 NLS (N terminal on insert)

SV40 NLS (C terminal on insert)

HA (C terminal on insert)