您的位置:首页 > 产品中心 > Anti-Neurophysin 2/NP-AVP Antibody, clone PS 41
Anti-Neurophysin 2/NP-AVP Antibody, clone PS 41
产品别名
Anti-Neurophysin 2/NP-AVP Antibody, clone PS 41
Vasopressin-neurophysin 2-copeptin, AVP-NPII, Arg-vasopressin, Arginine-vasopressin, Neurophysin 2, Neurophysin-I, Copeptin, Neurophysin 2/NP-AVP
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | The neurophysin 1 and 2 (NPI and NPII) are carriers of peptide hormone oxytocin (OT) and vasopressin (AVP), respectively. Each NP and its associated hormone peptide are synthesized as one precursor protein, OT-NPI (UniProt P01179) encoded by the Oxt (or Ot) gene or AVP-NPII (UniProt P01186) encoded by the Avp gene in rat species. Post-translational cleavages remove the signal peptide and process the precursor into mature OT and NPI (aa 20-28 and aa 32-135, respectively, of OT-NPI) or AVP and NPII (aa 24-32 and aa 36-128, respectively, of AVP-NPII). OT is a potent hormone and neurotransmitter secreted within the pituitary gland. OT exerts its biological activity through its receptor OXTR (also known as OTR). OT is used clinically for labor induction and may exhibit therapeutic potential for treating social anxiety disorders. AVP functions primarily as an anti-diuretic and regulates the concentration of water, glucose, and salts in blood. AVP is secreted from the posterior pituitary, but exerts its biological activity in the kidney via the G protein-coupled receptor AVPR2 (V2R). |
Specificity【特异性】 | Clone PS 41 reacts with Vasopressin-neurophysin 2-copeptin precursor, neurophysin 2, but not Arg-vasopressin, copeptin, oxytocin, or neurophysin 1. |
Immunogen【免疫原】 | KLH-conjugated acid soluble extract of rat (Sprague-Dawley) intermediate lobes. Epitope: Neurophysin 2 |
Application【应用】 | This Anti-Neurophysin 2/NP-AVP Antibody, clone PS 41 is validated for use in Western Blotting and Immunohistochemistry, Radioimmunoassay, Electron Microscopy, Immunoprecipitation and ELISA for the detection of Neurophysin 2/NP-AVP. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Neurophysin 2/NP-AVP in rat pituitary tissue. Immunohistochemistry Analysis: A representative lot detected Neurophysin 2/NP-AVP immunoreactivity in the hypothalamus of developing rats as early as embryonic day (E16) using coronal vibratome sections. (Witnall, M.H., et al.(1985). J Neurosci. 5(1):98-109). Radioimmunoassay Analysis: Target reactivity of a representative lot was confirmed by RIA using plates coated with purified rat Neurophysin/NP and 125I-labelled secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97). Electron Microscopy Analysis: A representative lot immunostained granulated neurosecretory vesicles (NSVs) in all axons, Herring bodies, and terminals by EM using rat posterior pituitary LR White sections (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97). Immunoprecipitation Analysis: A representative lot immunoprecipitated radiolabelled neurophysin 2, but not neurophysin 1 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97). ELISA Analysis: A representative lot detected neurophysin 2 immunoreactivity in rat neurophysin preparations by non-sandwich ELISA using either 125I-labelled or HRP-conjugated secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97). Research Category Neuroscience Research Sub Category Developmental Neuroscience |
Quality【质量】 | Evaluated by Western Blotting in rat pituitary tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Neurophysin 2/NP-AVP in 10 µg of rat pituitary tissue lysate. |
Physical form【外形】 | Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G Purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | PS 41, monoclonal |
species reactivity | rat |
technique(s) | ELISA: suitable electron microscopy: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable radioimmunoassay: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2bκ |
NCBI accession no.【NCBI登记号】 | NP_058688 |
UniProt accession no.【UniProt登记号】 | P01186 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~15 kDa observed |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |