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Anti-Neurofilament NF-H Antibody, clone TA51
产品编号: | 4110889 |
规格: | culture supernatant, clone TA51, Chemicon® |
包装规格: | 1 ML |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-Neurofilament NF-H Antibody, clone TA51
NF-H
基本信息
eCl@ss | 32160702 |
Specificity【特异性】 | Neurofilament H (NF-H), carboxy terminal (phosphate dependent (+). Clone is specific for phosphorylated NF-H {Carden et al (1987) 7(11):3489}. |
Immunogen【免疫原】 | Epitope: carboxy terminal phosphorylation dependent, within the multiphosphorylation repeat in NFH |
Application【应用】 | Anti-Neurofilament NF-H Antibody, clone TA51 is an antibody against Neurofilament NF-H for use in ELISA, IP, WB, IH. Research Category Neuroscience Research Sub Category Neurofilament & Neuron Metabolism Neuronal & Glial Markers Western blot: Identifies ~ 200kDa band in reduced samples. Use Tris based buffers for best results as epitope is phosphate dependent; Different regions of the CNS were dissected out immediately and homogenized in BUST buffer (8 M urea, 0.5% SDS, 2% 13-mercaptoethanol, and 50 mM Tris, pH 7.4) at a ratio of i ml/100 mg wet tissue. The tissue suspensions were sonicated well and centrifuged in a TL-100 ultracentrifuge (Beckman Instruments, Fullerton, CA) at 100,000 g for 1 h at 25°C, and the protein concentrations in the supernatants were determined. Proteins were separated on 7.5% SDS-PAGE gels, {Tu. P et al (1995) J Cell Biol 129:1629-1640} Immunohistochemistry on frozen sections; not effective on paraffin tissues in original work. {Carden et al, 1987}. Optimal working dilutions must be determined by end user. |
Physical form【外形】 | Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | rat |
antibody form【抗体形式】 | culture supernatant |
antibody product type | primary antibodies |
clone【克隆】 | TA51, monoclonal |
species reactivity | monkey, hamster, rat, human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG |
NCBI accession no.【NCBI登记号】 | NM_021076.2 |
UniProt accession no.【UniProt登记号】 | P12036 |
shipped in【运输】 | dry ice |
产品说明
Storage and Stability【储存及稳定性】 | Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |