Anti-Myc Tag Antibody, clone 4A6, HRP conjugate

32160702

NA.56

Immunogen: peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc

Specificity: Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

Species Cross-reactivity: Human, others not tested

Ig Type: IgG

Host: Mouse

Molecular Weight: Varies depending upon the protein which is tagged
Myc-Tag is a short synthetic peptide sequence derived from C-terminal region of human c-myc that is used as an epitope tag. The Myc tag can be cloned either upstream or downstream of the target gene, resulting in a fusion protein that allows efficient purification, detection, and localization of target proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. The myc epitope tag is widely used to detect expression of recombinant proteins in bacterial, yeast, insect, and mammalian systems.

Other species cross-reactivity not tested.
Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

Epitope: C-terminal
KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Clone 4A6.

Research Category
Epitope Tags & General Use
Anti-Myc Tag Antibody, clone 4A6, HRP conjugate detects level of Myc Tag & has been published & validated for use in WB.
Research Sub Category
Epitope Tags

Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected myc-PP2A on 10 µg of Myc-PP2A A subunit transfected NIH/3T3 lysates.

Purified mouse monoclonal IgG1 conjugated to horseradish peroxidase (HRP) in buffer containing: 50% storage buffer (PBS, 0.05% Kathon^®, 0.2 mg/mL BSA) and 50% glycerol.
Protein G Purified

Control
Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

peroxidase conjugate

purified immunoglobulin

primary antibodies

4A6, monoclonal

human

Upstate^®

western blot: suitable

NP_002458

P01106

dry ice

Varies depending upon the protein which is tagged.

Stable for 1 year at -20ºC from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS07,GHS09

Warning

H317 - H411

P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313

Aquatic Chronic 2 - Skin Sens. 1

10 - Combustible liquids

WGK 3

390.2 °F - Information refers to the main component.

199 ℃ - Information refers to the main component.