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Anti-Beta Galactosidase Antibody, E. coli
| 产品编号： | 4093911 |
| 规格： | from chicken, purified by affinity chromatography |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Beta Galactosidase Antibody, E. coli
Beta-galactosidase, Acid beta-galactosidase, Lactase, Elastin receptor 1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | β Galactosidase, also known as Beta-galactosidase, Beta-gal, LacZ, or Lactase, and encoded by the gene lacZ, is the enzyme in bacteria that hydrolyzes galactosides (sugars chains containing galactose) in to monosaccharides. β Galactosidase is typically an extracellularly produced enzyme that hydrolyzes the beta-glycosidic bond between galactose and another organic moiety. In E. coli, the β Galactosidase gene is present as part of the inducible lac operon system which is activated in the presence of lactose when glucose level is low. Today, bacterial beta galactosidase is used in molecular biology as a reporter marker to monitor gene expression, and because the enzyme has two functional domains, it forms the basis for α-complementation which is the methodology behind for the blue/white screening of recombinant clones. The presence or absence of an active β-galactosidase may be detected by X-gal, which produces a characteristic blue dye when cleaved by β-galactosidase, thereby providing an easy means of distinguishing the presence or absence of cloned product in a plasmid. |
| Immunogen【免疫原】 | Recombinant protein corresponding to E. Coli Beta Galactosidase. |
| Application【应用】 | Anti-Beta Galactosidase Antibody, E. coli is a highly specific chicken polyclonal antibody & that targets β-Galactosidase & has been tested in western blotting. |
| Quality【质量】 | Evaluated by Western Blotting in TOP10/PCU19 E. Coli lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Beta Galactosidase in 10 µg TOP10/PCU19 E. Coli lysate. |
| Linkage【联系】 | Replaces: AB3403 |
| Other Notes【其他说明】 | Affinity purified |
产品性质
| biological source【生物来源】 | chicken |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | bacteria |
| technique(s) | western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_752394 |
| UniProt accession no.【UniProt登记号】 | P16278 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~120 kDa observed |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
