Elastase Substrate V, Fluorogenic-CAS 72252-90-5-Calbiochem

C₃₁H₄₁N₅O₉

627.69

MFCD00077135

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte (K_m = 140 µM; K_cat/K_m = 120,000 s^-1 M^-1) and porcine pancreatic elastase. Not hydrolyzed by cathepsin G. Excitation max.: ~380 nm; emission max.: ~460 nm.
Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM; k_cat/K_m = 120,000 M^-1 s^-1). Not hydrolysed by cathepsin G.

Km = 140 µM; kcat/Km = 120,000 M⁻¹ s⁻¹as substrates for human leukocyte and porcine pancreatic elastase
Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase

Toxicity: Standard Handling (A)

MeOSuc-Ala-Ala-Pro-Val-AMC

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.

Barrett, A.J. 1981. Methods Enzymol. 80, 581.
Castillo, M.J., et al. 1979. Anal. Biochem.99, 53.
Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

200

≥95% (HPLC)
≥95% (TLC)

lyophilized solid
powder

Calbiochem^®

OK to freeze
desiccated
protect from light

white to off-white

ethanol: 1 mg/mL
DMF: 5 mg/mL
DMSO: 5 mg/mL

λ_ex 360-380 nm
λ_em 430-460 nm

ambient

−20℃

1S/C31H41N5O9/c1-16(2)27(34-20-9-10-21-17(3)14-26(39)45-23(21)15-20)30(42)35-29(41)22-8-7-13-36(22)31(43)19(5)33-28(40)18(4)32-24(37)11-12-25(38)44-6/h9-10,14-16,18-19,22,27,34H,7-8,11-13H2,1-6H3,(H,32,37)(H,33,40)(H,35,41,42)/t18-,19-,22-,27-/m0/s1

NHMQNKPYBPDCAY-NFHWZJRKSA-N

25 mg in Plastic ampoule

11 - Combustible Solids

WGK 3