Caspase-3 Substrate II, Fluorogenic-CAS 169332-61-0-Calbiochem

C₃₀H₃₇N₅O₁₃

675.64

MFCD00671411

Fluorogenic caspase-3 substrate. Cleavage of this substrate by caspase-3 shows Michaelis-Menten kinetics (K_m = 9.7 ± 1.0 µM). Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.
Fluorogenic CPP32/Apopain substrate. Cleavage of this substrate by CPP32/Apopain shows Michaelis-Menten kinetics (K_m = 9.7 ± 1.0 µM). Cleavage can be monitored by excitation at 380 nm and emission at 460 nm.

Product does not compete with ATP.
Reversible: no
Cell permeable: no
Km = 9.7 ± 1.0 µM for cleavage of this substrate by caspase-3
Primary Target
Fluorogenic caspase-3 substrate

Toxicity: Standard Handling (A)

Ac-Asp-Glu-Val-Asp-AMC

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Hermission, M., et al. 2000. Oncogene 19, 2338.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W., et al. 1995. Nature 376, 37.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

off-white

DMSO: 10 mM

λ_ex 365-380 nm
λ_em 430-460 nm

ambient

−20℃

1, 5 mg in Plastic ampoule

11 - Combustible Solids

WGK 3