Anti-Dog IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

MFCD00162265

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. In dogs four subclasses of IgG (IgG1 -IgG4) are present.
Dog IgGs are glycoprotein antibodies that regulate several immunological responses. Canine IgGs can be used as markers for detecting immune-mediated haemolytic anaemia, adverse food reactions and also for assessing the risk of L. infantum transmission in dogs.

Anti-Dog IgG (whole molecule) antibody is specific for IgG in dogs when tested against normal dog serum and dog IgG by double-diffusion and immunoelectrophoretic assays prior to conjugation.

IgG isolated from pooled normal dog serum

Anti-Dog IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA), and immunoblotting.

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide

rabbit

200

alkaline phosphatase conjugate

IgG fraction of antiserum

secondary antibodies

polyclonal

buffered aqueous solution

dog

direct ELISA: 1:6,000
dot blot: 1:4,000
western blot (chemiluminescent): 1:4,000

wet ice

2-8℃

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12 - Non Combustible Liquids

WGK 3