GranuCult^® Nutrient Broth

200

sterile; autoclavable

medium granules (dehydrated (DCM))

GranuCult^®

microbiological culture: suitable

7.0 (37 ℃, 8 g/L in H₂O, after autoclaving)

8 g/L

470 kg/m³

food and beverages
pharmaceutical

15-25℃

heterotrophic bacteria

GranuCult^® Nutrient Broth is designed as a basic medium to support the growth of fastidious and non-fastidious microbes in test samples.
The medium is supplemented with peptone and yeast extract contributing to the nitrogen requirements, vitamins and amino acids for the growth of microbes. Sodium Chloride maintains the osmotic balance glucose serves as the energy source.

GranuCult^® Nutrient Broth is designed as a basic medium to support the growth of fastidious and non-fastidious microbes in test samples.

Replaces: 1.05443

Appearance (clearness): clear
Appearance (color): yellowish to yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 100
Inoculum on reference medium (Streptococcus pyogenes ATCC 12344): ≤ 100
Inoculum on reference medium (Listeria monocytogenes ATCC 19118): ≤ 100
Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≤ 100
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): fair to very good
Growth (Streptococcus pyogenes ATCC 12344): fair to very good
Growth (Listeria monocytogenes ATCC 19118): fair to very good
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): fair to very good
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): fair to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): fair to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): fair to very good
Incubation: 24 ± 2 hours at 37 ± 1 °C, aerobic
Reference media: Tryptic Soy Agar

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 3