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GranuCult® Brain Heart Infusion broth

产品编号:4059292
规格:ISO 6888, To support the growth of fastidious and non-fastidious microorganisms
包装规格:500 G
产品类别:进口试剂
品牌:Millipore
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4059292500 G2330
产品性质
Quality Level【质量水平】
200
agency
APHA
ISO 6888
form【形式】
medium granules (dehydrated (DCM))
technique(s)
microbiological culture: suitable
pH【pH值(酸碱度)】
7.2-7.6 (37 ℃, 37 g/L in H2O, after autoclaving)
solubility【溶解性】
37 g/L
bulk density【堆积密度】
730 kg/m3
application(s)
food and beverages
microbiology
pathogen testing
storage temp.【储存温度】
15-25℃
suitability【适用性】
heterotrophic bacteria
基本信息
General description【一般描述】
BHI (Brain Heart Infusion) broth is a granulated selective medium recommended for the cultivation of various fastidious bacteria and the cultivation of Staphylococci for the plasma coagulase test. The granules ensure rapid and uniform dissolution in water. It also prevents clumping of the medium and inhalation of the airborne powder. Enzymatic digest of animal tissues and infusions (calf brain and beef heart) serve as sources of carbon, nitrogen, essential growth factors, amino acids, and vitamins. Glucose serves as a source of energy. Disodium phosphate helps in maintaining the buffering action of the medium, whereas sodium chloride maintains the osmotic equilibrium of the medium.
Application【应用】
BHI (Brain Heart Infusion) broth is a highly nutritious and buffered medium to support the growth of fastidious and non-fastidious microorganisms, including aerobic and anaerobic bacteria.
Analysis Note【分析说明】
Appearance (colour): brown
Appearance (clearness): clear
pH-value (25 °C): 7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 100
Inoculum on reference medium (Streptococcus pneumoniae ATCC 6305): ≤ 100
Inoculum on reference medium (Streptococcus pyogenes ATCC 19615): ≤ 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≤ 100
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): ≤ 100
Inoculum on reference medium (Bacteroides fragilis ATCC 25285): ≤ 100
Inoculum on reference medium (Haemophilus influenzae ATCC 33391): ≤ 100
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): good to very good
Growth (Streptococcus pneumoniae ATCC 6305): good to very good
Growth (Streptococcus pyogenes ATCC 19615): good to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): good to very good
Growth (Candida albicans ATCC 10231 (WDCM 00054)): good to very good
Growth (Bacteroides fragilis ATCC 25285): good to very good
Growth (Haemophilus influenzae ATCC 33391): good to very good
Incubation:
24 ± 2 hours at 37 ± 1 °C aerobic
C.albicans 44 ± 4 hours at 37 ± 1 °C aerobic
B.fragilis 44 ± 4 hours at 37 ± 1 °C anaerobic
H.influenza 44 ± 4 hours at 37 ± 1 °C microaerophilic and with addition of X+V-factor.
Footnote【附注】
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information【法律信息】
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
安全信息
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 3
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