Anti-GADD153 antibody produced in rabbit

MFCD03455044

NA.41

Growth arrest and DNA damage inducible protein 153, C/EBP homology protein (GADD153/CHOP10) is a basic region leucine zipper transcription factor, devoid of DNA binding site. Its apparent molecular mass is 29 kDa (or 19 kDa calculated).

peptide corresponding to human GADD153/CHOP10 sequence (amino acids 153-169) with N-terminal lysine added, conjugated to keyhole limpet hemocyanin (KLH). The corresponding sequence in mouse and rat differs by 2 amino acids.

Anti-GADD153 antibody produced in rabbit is suitable as a primary antibody in western blot analysis of:

• nuclear and cytoplasmic fractions extracted from rat insulinoma (INS) cells to study CHOP Expression in response to ER stress
• whole cell lysates prepared from ER stress induced rat pancreatic tissues
• whole cell lysates prepared from ER stress induced pancreatic insulin-producing cells

It is suitable for immunocytochemistry at a working concentration of 5-10μg/mL using Hela human cell and microarray. It is also suitable for western blotting at a working dilution of 1:200-1:400 using whole cell extract of HEK-293 over-expressing GADD 153.

GADD153/CHOP10 (Growth Arrest and DNA Damage Inducible Protein 153, C/EBP Homology Protein) is a leucine zipper transcription factor that lacks a DNA binding site. The protein homodimerizes with members of the CCAAT/enhancer binding protein (C/EBP) family of transcription factors during adipogenesis. Many signals induce the expression of CHOP10. Some of these signals include endoplasmic reticulum stress, hyperosmosis, growth arrest, DNA damage, calcium onophore, nutrient deprivation, hypoxia and hyperoxia. GADD153 heterodimerizes with C/EBPb and inhibits its binding to DNA sequences. Expresssion of this gene induces growth arrest in fibroblasts, inhibits adipocyte differentiation, and is associated with apoptosis in vitro. GADD153 binds to several nuclear proteins as well as the FTE/S3a ribosomal protein and enhances erythroid differentiation. It negatively regulates ATF3, a stress-inducible transcriptional repressor.
Growth arrest and DNA damage inducible protein 153, C/EBP homology protein (GADD153/CHOP10) is also involved in apoptosis in lungs of mice exposed to hyperoxia.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen 29 kDa

human

immunocytochemistry: 5-10 μg/mL using Hela human cell
microarray: suitable
western blot (chemiluminescent): 1:200-1:400 using whole cell extract of HEK-293 over-expressing GADD 153.

P35638

dry ice

−20℃

human ... DDIT3(1649)
rat ... Ddit3(29467)

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