Sialyltransferase Inhibitor, 3Fax-Peracetyl Neu5Ac-Calbiochem

C₂₂H₃₀FNO₁₄

551.47

A cell-permeable sialylic acid analog that upon cellular uptake is transformed into a CMP-Neu5Ac (Cat. No. 233264) mimetic bearing a C3 fluorine substituent at the axial position, effectively inhibiting sialyltransferase in a donor substrate CMP-Neu5Ac-competitive manner. Shown to effectively abloishes HL-60 cell surface SLe^X expression (by >95%; 200 µM for 5 days), resulting in dramatic reductions in cell surface E-selectin and P-selectin binding (by >95% and >80%, respectively), without affecting cell viability or proliferation.
A cell-permeable sialylic acid analog that upon cellular uptake is transformed into a CMP-Neu5Ac (a href="http://www.emdmillipore.com/products/EMD_BIO-233264" target=_blank>233264) mimetic bearing a C3 fluorine substituent at the axial position, effectively inhibiting sialyltransferase in a donor substrate CMP-Neu5Ac-competitive manner. Shown to effectively abloishes HL-60 cell surface SLe^X expression (by >95%; 200 µM for 5 days), resulting in dramatic reductions in cell surface E-selectin and P-selectin binding (by >95% and >80%, respectively), without affecting cell viability or proliferation.

Cell permeable: yes
Primary Target
Sialyltransferase
Reversible: yes

Toxicity: Standard Handling (A)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -70°C.

Rillahan, C., et al. 2012. Nat. Chem. Bio.8, 661.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (NMR)

solid

Calbiochem^®

OK to freeze
protect from light

white

DMSO: 50 mg/mL

ambient

−70℃

F[C@@H]1[C@@H]([C@H](C([C@@H]([C@@H](COC(C)=O)OC(C)=O)OC(C)=O)OC(C(OC)=O)1OC(C)=O)NC(C)=O)OC(C)=O

10 mg in Glass bottle
Packaged under inert gas

11 - Combustible Solids

WGK 2