MLK3 (1-488), active, GST tagged human

NA.32

MLK3 or Mixed-Lineage Kinase 3 is a mitogen-activated protein kinase kinase kinase capable of preferentially activating MAPK8/JNK kinase and functions as a positive regulator of JNK signaling pathway. MLK3 can directly phosphorylate and activate IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. MLK3 is a signal-integrating kinase with conventional MAP3K catalytic activity and additional noncatalytic functions that contribute to RAF/ERK signalling. MLK3 is a component of the BRAF/RAF1 complex and is required for integrity of the complex and for activation of ERK by the complex.

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

after opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

human

expressed in baculovirus infected Sf9 cells

PRECISIO^® Kinase

≥70% (SDS-PAGE)

buffered aqueous glycerol solution

8-11 nmol/min·mg

~83 kDa

NM_002419

cell analysis

dry ice

−70℃

human ... MAP3K11(4296)

10 - Combustible liquids

WGK 1