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JMJD2b active human
| 产品编号： | 4119616 |
| 规格： | recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE) |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
JMJD2b active human
KDM4B
lysine (K)-specific demethylase 4B
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015), amino acids 2-500 with N-terminal GST-tag, MW=83 kDa, expressed in Sf9 cells using a Baculovirus expression system. |
| Specificity【特异性】 | 0.32 pmole/min/μg Assay conditions: 10μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 μM a-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween20, 0.5 μM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin conjugated secondary antibody followed by Alpha Screening detection. |
| Application【应用】 | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Physical form【外形】 | Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% Glycerol. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in baculovirus infected Sf9 cells |
| Assay【测定】 | ≥70% (SDS-PAGE) |
| form【形式】 | aqueous solution |
| mol wt【分子量】 | 83 kDa |
| packaging【包装】 | pkg of 100 μg |
| NCBI accession no.【NCBI登记号】 | NM_015015 |
| UniProt accession no.【UniProt登记号】 | O94953 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... KDM4B(23030) |
产品说明
| Storage and Stability【储存及稳定性】 | At least 6 months at –80℃. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%). |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
