p21-Activated Kinase Inhibitor III, IPA-3-CAS 42521-82-4-Calbiochem

C₂₀H₁₄O₂S₂

350.45

MFCD00388917

A cell-permeable symmetrical disulfide allosteric inhibitor that selectively targets the autoregulatory domain of group I, but not group II, PAKs (p21-activated kinases) and prevents the activation of PAK1/2/3 (% inhibition= 95, 70, and 60, respectively, with 10 µM inhibitor; IC₅₀ = 2.5 µM for PAK1). Specificity test shows ≥ 50% inhibition, in the presence of 10 µM ATP and inhibitor, against 9 of 214 non-PAK human kinases. Shown to effectively suppress both basal and PDGF-induced PAK activation at 30 µM in mouse embryonic fibroblasts. IPA-3 does not inhibit the enzymatic activity of preactivated PAKs and reduction of the intramolecular disulfide bond of IPA-3 by DTT renders it inactive.

Toxicity: Irritant (B)

Deacon, S.W., et al. 2008. Chem. Biol.15, 322.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥97% (HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

yellow

DMSO: 10 mg/mL
ethanol: 2 mg/mL

wet ice

−20℃

5, 25 mg in Plastic ampoule
Packaged under inert gas

GHS05,GHS09

Danger

H318 - H410

P273 - P280 - P305 + P351 + P338 - P391 - P501

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1

11 - Combustible Solids

WGK 3