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Nek2, Active human
| 产品编号： | 4084398 |
| 规格： | recombinant, expressed in E. coli, ≥85% (SDS-PAGE), buffered aqueous glycerol solution |
| 包装规格： | 5 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Nek2, Active human
Nima-Related Kinase 2
Never In Mitosis Gene A-Related Kinase 2
基本信息
| MDL number | MFCD07370971 |
| NACRES | NA.32 |
| Application【应用】 | Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 ℃ using a final concentration of 50 μM [32P] ATP. |
| Biochem/physiol Actions【生化/生理作用】 | Nek2 (nimA-related kinase 2) is a cell cycle-regulated protein kinase with maximal activity at the onset of mitosis that localizes to the centrosome. |
| Unit Definition【单位定义】 | One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP |
| Physical form【外形】 | Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol. |
产品性质
| Quality Level【质量水平】 | 200 |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | ≥85% (SDS-PAGE) |
| form【形式】 | buffered aqueous glycerol solution |
| enzyme activity | ≥10 units/mg protein |
| mol wt【分子量】 | protein apparent mol wt ~80 kDa |
| UniProt accession no.【UniProt登记号】 | P51955 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... NEK2(4751) |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
