MAP Kinase 1, His·Tag^®, Human, Recombinant, E. coli

≥95% (SDS-PAGE)

100

liquid

≥6000 units/mg protein

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

Proteases, none detected

wet ice

−70℃

2 μg in Plastic ampoule

Recombinant, human MAP Kinase 1 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
Recombinant, human MAP Kinase 1 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of purified, recombinant MAP kinase in vitro with MEK1. Suitable for labeling MAP kinase substrates or for inhibitor screening.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.

In 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.

Following initial thaw, aliquot and freeze (-70°C).

Charest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

10 - Combustible liquids

WGK 1