Calpain-1 Substrate, Fluorogenic-Calbiochem

C₇₉H₉₅N₁₃O₁₉S₂

1594.81

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (K_m = 4.6 µM; k_cat = 11 s^-1). Cleavage occurs between Tyr-Gly residues and results in enhanced fluorescence.
An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (K_m = 4.6 µM; k_cat = 11 s^-1). Cleavage occurs at the Tyr-Gly bond and results in enhanced fluorescence. Excitation max: ~490 nm; emission max: ~518 nm.

Product does not compete with ATP.
Primary Target
A sensitive and specific substrate for calpain-1
Reversible: no
Cell permeable: no

Toxicity: Standard Handling (A)

H-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH

Supplied as a trifluoroacetate salt.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Mittoo, S., et al. 2003. Anal. Biochem.319, 234.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (HPLC)

lyophilized solid

Calbiochem^®

OK to freeze
desiccated (hygroscopic)
protect from light

red

DMSO: 1 mg/mL

λ_ex ~490 nm
λ_em ~518 nm

wet ice

−20℃

2 mg in Plastic ampoule
Packaged under inert gas

11 - Combustible Solids

WGK 1