Rapid Cell Proliferation Kit

100

sufficient for 500 tests

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

sample type cultured cells

sample: 1000 - 50,000 cells

colorimetric

wet ice

−20℃

1 kit in Glass bottle

Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.

Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)

WST-1, 1-Methoxy PMS, and a user protocol.

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Assay Time: 0.5 - 4 h

The Calbiochem Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.

• WST-1 Labeling Mixture: Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.

Ishiyama, M., et al. 1995. In Vitro Toxicology8, 187.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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