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Polyphosphoinositide-Binding Peptide, PBP10-Calbiochem
| 产品编号： | 4061899 |
| 规格： | A Rhodamine-B-labeled decapeptide derived from the (PIP2)-binding region in segment-2 of gelsolin. |
| 包装规格： | 1 MG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Polyphosphoinositide-Binding Peptide, PBP10-Calbiochem
RhoB-GS 160-169, Rhodamine B-QRLFQVKGRR-OH
RhoB-GS160-169, Rhodamine B-QRLFQVKGRR-OH
基本信息
| Empirical Formula【经验(实验)分子式】 | C84H127N24O15 |
| Molecular weight | 1713.06 |
| General description【一般描述】 | A Rhodamine-B-labeled decapeptide derived from the (PIP2)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelet without significantly affecting intracellular Ca2+ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs. A Rhodamine-B-labeled decapeptide derived from the (PIP2)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelets without significantly affecting intracellular Ca2+ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs. Excitation max.: ~565 nm; emission max.: ~590 nm. |
| Biochem/physiol Actions【生化/生理作用】 | Product does not compete with ATP. Primary Target Specifically binds to polyphosphoinositide (PPI) Reversible: yes Cell permeable: yes |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Sequence【序列】 | Rho-B-Gln-Arg-Leu-Phe-Gln-Val-Lys-Gly-Arg-Arg-OH |
| Physical form【外形】 | Supplied as a trifluoroacetate salt. |
| Reconstitution【重悬】 | Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. |
| Other Notes【其他说明】 | Bucki, R., et al. 2001. Biochemistry40, 15752 Cunningham, C.C., et al. 2001. J. Biol. Chem.276, 43390. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 100 |
| Assay【测定】 | ≥95% (HPLC) |
| form【形式】 | lyophilized solid |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze desiccated (hygroscopic) protect from light |
| color【颜色】 | pink-red |
| solubility【溶解性】 | DMSO: 2 mg/mL |
| fluorescence【荧光】 | λex 565 nm λem 590 nm |
| shipped in【运输】 | ambient |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 1 mg in Glass bottle Packaged under inert gas |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 1 |
