DAR-4M AM-Calbiochem

C₂₈H₃₁IN₄O₅

630.47

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH above 4.0. Excitation max: ~560 nm; emission max: ~575 nm.

Product does not compete with ATP.
Cell permeable: yes
Primary Target
Nitric oxide (NO) fluorescent indicator
Reversible: no

Toxicity: Irritant (B)

In DMSO.

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (HPLC)

solution

Calbiochem^®

OK to freeze
protect from light

dark red

λ_ex ~560 nm
λ_em ~575 nm

ambient

−20℃

1 mg in Plastic ampoule
Packaged under inert gas

10 - Combustible liquids

WGK 2