Carboxypeptidase B, Porcine Pancreas

3.4.17.2 ( BRENDA | IUBMB )

MFCD00081476

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides. Inhibited by EDTA and other Zn²⁺ chelators.
Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides. Inhibited by EDTA and other Zn²⁺ chelators. Has an optimal pH of 7.0-9.0 and a pI of 6.0.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Hip-Arg per min at 25°C, pH 7.7.

Lyophilized from 360 µM HEPES, 1.41 µM zinc chloride, 1.41 mg/ml Trehalose, pH 7.4.

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Folk, J.E. 1970. Methods Enzymol.19, 504.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

lyophilized

≥100 units/mg protein

Calbiochem^®

OK to freeze

6.0

water: 1 mg/mL

Carboxypeptidase A <1.0%
Chymotrypsin, none detected
Trypsin ≤5%

ambient

−20℃

1 mg in Plastic ampoule

GHS07,GHS08

Danger

H315 - H319 - H334 - H335

P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Respiratory system

10 - Combustible liquids

WGK 3