β-Secretase Substrate IV, Fluorogenic-Calbiochem

C₉₁H₁₂₉N₂₅O₂₅S

2005.22

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in fluorescence enhancement. Reported K_cat/k_m values (min^-1/µM) for pro-mempasin 2 and its proteolytically activated species Leu^28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37°C).
A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement. k_cat/K_m values (min^-1/µM) at 37°C, pH 4.5, for pro-memapsin 2 and its activated species Leu^28p-memapsin 2 are 0.044 and 0.040, respectively.

Product does not compete with ATP.
Primary Target
β-secretase substrate
Reversible: no
Cell permeable: no

Toxicity: Standard Handling (A)

H-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH

Supplied as a trifluoroacetate salt.

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.

Ermolieff, J., et al. 2000. Biochemistry 39, 12450.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (HPLC)

lyophilized solid

Calbiochem^®

OK to freeze
protect from light

red

DMSO: 5 mg/mL

λ_ex ~350 nm
λ_em ~490 nm

ambient

−20℃

1 mg in Plastic ampoule
Packaged under inert gas

11 - Combustible Solids

WGK 1