Topoisomerase I, His·Tag^®, Human, Wild Type, Recombinant, S. frugiperda

≥95% (SDS-PAGE)

100

liquid

1 units/ng protein

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−70℃

1 set in Glass bottle
Please refer to vial label for lot-specific concentration.

Recombinant, human topoisomerase I fused to a His•Tag sequence and expressed in S. frugiperda insect cells. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. DNA Topo I is an important target for ant-tumor agents as a result of the fact that is causes single-strand DNA breaks of the type that are present in most cancers.
Recombinant, human topoisomerase I fused to a His•Tag sequence and expressed in S. frugiperda insect cells. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. Since Topo I can cause large amounts of single-strand DNA breaks it is an important target for antitumor agents. One unit= 1 ng protein.

0.1-1 unit (ng) is sufficient for relaxing 0.5 µg of pBR322 supercoiled DNA in a 20 µl reaction.

Toxicity: Standard Handling (A)

5000 U enzyme in 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 7.9 and 1 vial dilution buffer (100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0).

Following initial thaw aliquot and freeze (-70°C). For storage of diluted protein, dilute in storage buffer with 500µg/ml molecular biology grade BSA, aliquot, and freeze (-70°C).

Pommier, Y., et al. 1998. Biochim. Biophys. Acta1400, 83.
Pourquier, P., et al. 1997. J. Biol. Chem.272, 26441.
Rossi, F., et al. 1996. Nature381, 80.
Sekiguchi, J., et al. 1996. Proc. Natl. Acad. Sci. USA93, 785.
Giovanella, B.C., et al. 1989. Science246, 1046.
Wang, J,C. 1985. Annu. Rev. Biochem.54, 665.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

10 - Combustible liquids

WGK 1