MDM2 Antagonist, Nutlin-3, Racemic-CAS 548472-68-0-Calbiochem

C₃₀H₃₀Cl₂N₄O₄

581.49

MFCD07784509

A cell-permeable cis-imidazoline compound that acts as a potent and selective MDM2 antagonist (IC₅₀ = 90 nM for Nutlin-3a and 13.6 µM for Nutlin-3b) and displays antitumor properties. Activates p53 pathway by binding MDM2 in the p53-binding pocket and inhibits MDM2-p53 interaction. Shown to suppress tumor growth by induction of apoptosis in several cancer cells with wild-type p53 and in xenografted tumor mice. A 10 mM (1 mg/172 µl) solution of MDM2 Antagonist, Nutlin-3, Racemic (Cat. No. 444151) in DMSO is also available.
A cell-permeable, potent, and selective MDM2 antagonist (IC₅₀ = 90 nM for Nutlin-3a and 13.6 µM for Nutlin-3b) that displays antitumor properties. Activates the p53 pathway by binding MDM2 in the p53-binding pocket and inhibiting the MDM2-p53 interaction. Shown to induce apoptosis in several cancer cell lines with wild-type p53 and in xenografted tumor mice.

Cell permeable: yes
Primary Target
MDM2
Target IC₅₀: 90 nM for Nutlin-3a and 13.6 µM for Nutlin-3b
Product does not compete with ATP.
Reversible: no

Toxicity: Harmful (C)

Following reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Thompson, T., et al. 2004. J. Biol. Chem.279, 53015.
Vassilev, L.T., et al. 2004. Science303, 844.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (TLC and HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

DMSO: 25 mg/mL
ethanol: 25 mg/mL

wet ice

−20℃

1S/C30H30Cl2N4O4/c1-18(2)40-25-16-23(39-3)12-13-24(25)29-34-27(19-4-8-21(31)9-5-19)28(20-6-10-22(32)11-7-20)36(29)30(38)35-15-14-33-26(37)17-35/h4-13,16,18,27-28H,14-15,17H2,1-3H3,(H,33,37)

BDUHCSBCVGXTJM-UHFFFAOYSA-N

1 mg in Plastic ampoule
Packaged under inert gas

11 - Combustible Solids

WGK 3