InSolution PD 98059-CAS 167869-21-8-Calbiochem

C₁₆H₁₃NO₃

267.28

MFCD00671789

Selective and cell-permeable inhibitor of MAP kinase kinase (MEK) that acts by inhibiting the activation of MAP kinase and subsequent phosphorylation of MAP kinase substrates. Pretreatment of PC-12 cells with PD 98059 completely blocks the 4-fold increase of MAP kinase activity produced by nerve growth factor (NGF; IC₅₀ = 2 µM); however, it has no effect on NGF-dependent tyrosine phosphorylation of the pp140^trk receptor or its substrate Shc and does not block NGF-dependent activation of PI 3-kinase. Inhibits cell growth and reverses the phenotype of ras-transformed BALB 3T3 mouse fibroblasts and rat kidney cells.

Cell permeable: yes
Target IC₅₀: 2 µM in completely blocking the 4-fold increase in MAP kinase activity produced by nerve growth factor in PC12 cells
Primary Target
MAP kinase kinase (MEK)
Product does not compete with ATP.
Reversible: yes

Toxicity: Irritant (B)

A 5 mg/ml solution of PD 98059 (Cat. No. 513000) in anhydrous DMSO.

Following initial thaw, aliquot and freeze.

Kültz, D., et al. 1998. J. Biol. Chem.273, 13645.
Dudley, D.T., et al. 1995. Proc. Natl. Acad. Sci. USA92, 7686.
Langlois, W.J., et al. 1995. J. Biol. Chem.270, 25320.
Pang, L., et al. 1995. J. Biol. Chem.270, 13585.
Waters, S.B., et al. 1995. J. Biol. Chem.270, 20883.



Selected Citations
Lee, J., et al. 2009. Cell Stem Cell5, 76.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (HPLC)

liquid

Calbiochem^®

OK to freeze
protect from light

wet ice

−20℃

1S/C16H13NO3/c1-19-14-8-4-6-11(16(14)17)15-9-12(18)10-5-2-3-7-13(10)20-15/h2-9H,17H2,1H3

QFWCYNPOPKQOKV-UHFFFAOYSA-N

1 ml in Glass bottle
Packaged under inert gas

10 - Combustible liquids

WGK 2