Indirubin-3'-monoxime-CAS 160807-49-8-Calbiochem

C₁₆H₁₁N₃O₂

277.28

MFCD02683594

A potent cell-permeable, reversible, and ATP-compatible inhibitor of glycogen synthase kinase-3β (IC₅₀ = 22 nM) and cyclin-dependent kinases (IC₅₀ = 180 nM for Cdk1 and 100 nM for Cdk5). Inhibits the proliferation of a large range of cells by arresting them in the G₂/M phase of the cell cycle. Inhibits Tau phosphorylation in vitro and in vivo at Alzheimer′s disease-specific sites. Also reported to inhibit the in vivo phosphorylation of DARPP-32 by Cdk5 on Thr⁷⁵.
A potent, reversible, and ATP-compatible inhibitor of GSK-3β (glycogen synthase kinase 3β), Cdk1 (cyclin-dependent kinase1) and Cdk5 (IC₅₀ = 22 nM, 180 nM, and 100 nM, respectively). Inhibits the proliferation of a large range of cells by arresting them in the G₂/M phase of the cell cycle. Inhibits Tau phosphorylation in vitro and in vivo at Alzheimer’s disease-specific sites. Also reported to inhibit the in vivo phosphorylation of DARPP-32 by Cdk5 on Thr⁷⁵.

Cell permeable: yes
Target IC₅₀: 22 nM, 180 nM, and 100 nM against GSK-3β, Cdk1, and Cdk5, respectively
Primary Target
Gsk-3β
Product competes with ATP.
Reversible: yes

Toxicity: Standard Handling (A)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Bain, J., et al. 2003. Biochem. J. 371, 199.
Leclerc, S., et al. 2000. J. Biol. Chem.276, 251.
Hoessel, R., et al. 1999. Nat. Cell Biol. 1, 60.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

dark red

DMSO: 5 mg/mL

ambient

−20℃

1 mg in Plastic ampoule
Packaged under inert gas

11 - Combustible Solids

WGK 3