γ-Secretase Substrate, Fluorogenic-Calbiochem

C₇₀H₁₁₆N₂₆O₁₈

1609.83

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ₄₀-, Aβ₄₂-, and Aβ₄₃-generating cleavage sites results in enhanced fluorescence.
An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ₄₀-, Aβ₄₂-, and Aβ₄₃-generating cleavage sites results in enhanced fluorescence. Excitation max.: ~355 nm; emission max.: ~440 nm.

Product does not compete with ATP.
Reversible: no
Cell permeable: no

Toxicity: Standard Handling (A)

NMA-Gly-Gly-Val-Val-Ile-Ala-Thr-Val-Lys(DNP)-D-Arg-D-Arg-D-Arg-NH₂

Following reconstitution, aliquot, flush with a stream of inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Farmery, M.R., et al. 2003. J. Biol. Chem.278, 24277.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (HPLC)

lyophilized

Calbiochem^®

OK to freeze
desiccated (hygroscopic)
protect from light

yellow

DMSO: 5 mg/mL
5% acetic acid: soluble

ambient

−20℃

1 mg in Plastic ampoule
Packaged under inert gas

11 - Combustible Solids

WGK 1