ERK Activation Inhibitor Peptide I, Cell-Permeable-Calbiochem

C₈₆H₁₅₃N₁₉O₁₇S

1757.32

A cell-permeable, reversible, and stearated, 13-amino acid peptide corresponding to the N-terminus of MAPK/ERK kinase (MEK). Acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of ELK1. Selectively binds to ERK2 and prevents its interaction with MEK (IC₅₀ = 2.5 µM). Shown to block ERK activity in PMA-stimulated NIH3T3 cells and NGF-treated PC12 cells (IC₅₀ = 13 µM). Does not affect the activation of either JNKs or p38 MAP kinase.
A cell-permeable and reversible stearated 13-amino acid peptide corresponding to the N-terminus of MEK1 (MAPKK) that acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of Elk1. Selectively binds to ERK2 and prevents its interaction with MEK (IC₅₀ = 2.5 µM). Potently blocks ERK activation in PMA-stimulated NIH 3T3 cells and NGF-treated PC12 cells (IC₅₀ = 13 µM). Does not affect the activation of JNKs or p38.

Primary Target
MEK
Product does not compete with ATP.
Reversible: yes
Cell permeable: yes

Toxicity: Standard Handling (A)

Ste-Met-Pro-Lys-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Pro-NH₂ (Ste = stearated)

Supplied as a trifluoroacetate salt.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Kelemen, B.R., et al. 2002. J. Biol. Chem.277, 8741.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (HPLC)

lyophilized solid

2.5 μM IC₅₀

Calbiochem^®

OK to freeze
desiccated (hygroscopic)
protect from light

white

water: 1 mg/mL
DMSO: 5 mg/mL

wet ice

−20℃

1 mg in Plastic ampoule
Packaged under inert gas

11 - Combustible Solids

WGK 1