Calmodulin, Porcine Brain

100

>98% (SDS-PAGE)

lyophilized

≥35,000 units/mg protein

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

water: 1 mg/mL

wet ice

−20℃

1 mg in Plastic ampoule

Calmodulin (CaM) purified from porcine brain. A ubiquitous, calcium-binding protein that interacts with and regulates a wide variety of proteins, thus affecting different cellular functions and calcium-calmodulin-dependent signaling pathways. CaM mediates processes like inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth, and the immune response. Useful for applications requiring active pure calmodulin.

Toxicity: Standard Handling (A)

One unit is defined as the amount of calmodulin that will stimulate 0.01 unit of 3′5′-cyclic nucleotide phosphodiesterase to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 µM Ca²⁺ in a 3 ml reaction volume at 30°C, pH 7.5.

Lyophilized from 10 mM sodium phosphate buffer, 5% sucrose, pH 7.0.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Massom, L., et al. 1990. Biochemistry29, 671.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 1