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产品性质
| biological source【生物来源】 | hamster kidney |
| growth mode【生长模式】 | Suspension |
| karyotype【核型】 | 2n = 44, pseudodiploid |
| morphology【形态学】 | Not specified |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Hamster Syrian kidney |
| Cell Line Description【细胞系描述】 | Derived from the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells have been adapted to growth in suspension and have the same virus susceptibility as the parent line. |
| Application【应用】 | Virus replication studies |
| Culture Medium【培养基】 | GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Maintain cultures between 2-9x100,000 cells/ml (split ratio of 1:3 to 1:6); 5% CO2; 37℃. Cells need to be grown in a techni-stirrer or they may start to re-attach. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
