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产品性质
| biological source【生物来源】 | bovine cardiovascular |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | 59 or 60 |
| morphology【形态学】 | Endothelial |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Bovine aortic endothelium |
| Cell Line Description【细胞系描述】 | Derived from the aorta of a Friesian cow. Cells form "sprouts" after confluence. Infected with Bovine Viral Diarrhoea Virus (BVDV) which is a common contaminant of ruminants and is non-hazardous to humans. Secretes proteoglycans and collagen. |
| Culture Medium【培养基】 | DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37℃. There is a tendancy for the cells to grow fibroblast-like until they are confluent. Once confluent the endothelial morphology prevail |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
