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产品性质
| biological source【生物来源】 | mouse brain |
| growth mode【生长模式】 | Aggregates in suspension |
| karyotype【核型】 | Diploid |
| morphology【形态学】 | Small round cells growing as large aggregates |
| products【产品】 | Adrenocorticotropic hormone (ACTH) |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Mouse LAF1 pituitary gland tumour |
| Cell Line Description【细胞系描述】 | Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week. |
| Application【应用】 | Hormone studies |
| Culture Medium【培养基】 | Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates. |
| Subculture Routine【传代培养常规】 | Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
