Anti-DFF45, C-Terminal antibody produced in rabbit

MFCD02096209

NA.41

DFF exists as an inactive cytoplasmic protein until activated by apoptotic signals. DFF40, the nuclease responsible for DNA fragmentation during apoptosis, exists as a heterodimeric complex with DFF45.

synthetic peptide corresponding to amino acids 313-331 of the C-terminal region of human DFF45/ICAD (DNA Fragmentation Factor 45/ Inhibitor of Caspase-3 Activated DNase I).

Anti-DFF45 antibody produced in rabbit is suitable for western blot analysis at a working concentration of 0.25μg/mL using human HeLa cell extract.

DFF45 functions as both a chaperone, mediating the correct folding of DFF40, and an inhibitor of DFF40. DFF45 is cleaved by caspase-3 at two sites in response to apoptotic signals. This releases DFF40, the active nuclease. DFF40 seems to oligomerize, forming a large, functional complex which breaks down DNA by introducing double strand breaks. Also, DFF40 appears to interact directly with histone H1 that may stimulate its activity.

Solution in phosphate buffered saline containing 0.02% sodium azide

rabbit

200

unconjugated

IgG fraction of antiserum

primary antibodies

polyclonal

buffered aqueous solution

antigen 45 kDa (non-apoptotic cells)

human

western blot: 0.25 μg/mL using human HeLa cell extract

O00273

dry ice

−20℃

human ... DFFA(1676)