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Anti-CDK4 antibody produced in rabbit
产品别名
Anti-CDK4 antibody produced in rabbit
Anti-PSK-J3
Anti-CMM3
基本信息
| NACRES | NA.41 |
| General description【一般描述】 | The antibody detects endogenous levels of total CDK4 protein. |
| Immunogen【免疫原】 | Peptide sequence around aa. 295-299 (L-H-K-D-E), according to the protein NP_000066.1 |
| Application【应用】 | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Features and Benefits【特点和优势】 | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Physical form【外形】 | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
产品性质
| biological source【生物来源】 | rabbit |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | ~34 kDa |
| species reactivity | human |
| concentration【浓度】 | 1 mg/mL |
| technique(s) | western blot: 1:500-1:1000 |
| isotype【同位素/亚型】 | IgG |
| immunogen sequence【免疫原序列】 | (L-H-K-D-E) |
| NCBI accession no.【NCBI登记号】 | NM_000075.2 |
| UniProt accession no.【UniProt登记号】 | P11802 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... CDK4(1019) |
产品说明
| Target description【目标描述】 | Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle . |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
