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Pyruvate Carboxylase from bovine liver
产品别名
9014-19-1
Pyruvate Carboxylase from bovine liver
Pyruvate:CO2 ligase (ADP-forming)
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 6.4.1.1 ( BRENDA | IUBMB ) |
| MDL number | MFCD00132143 |
| NACRES | NA.26 |
| Application【应用】 | Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes. The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum. |
| Biochem/physiol Actions【生化/生理作用】 | Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA. |
| Unit Definition【单位定义】 | One unit will convert 1.0 μmole of pyruvate and CO2 to oxalacetate per min at pH 7.8 at 30 ℃. |
| Physical form【外形】 | Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA. |
| Preparation Note【制备说明】 | 亲和纯化 |
产品性质
| form【形式】 | buffered aqueous glycerol solution |
| Quality Level【质量水平】 | 200 |
| Specific Activity | 5-25 units/mg protein (BCA) |
| concentration【浓度】 | ≥0.5 mg/mL |
| foreign activity【异质活性】 | lactic dehydrogenase ≤0.5% |
| storage temp.【储存温度】 | −20℃ |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
