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Pyrophosphate reagent
基本信息
| NACRES | NA.54 |
| Application【应用】 | The product has been used for the measurement of pyrophosphate content of plant tissues. It has also been used as a rapid, routine check of pyrophosphate content by monitoring NADH oxidation in Solanum tuberosum tuber extracts. |
| Biochem/physiol Actions【生化/生理作用】 | The pyrophosphate detection kit couples the production of pyrophosphate to the consumption of NADH. |
| Components【组分】 | ComponentConcentration Imidazole × HCl, pH 7.4 45 mM Citrate 5 mM EDTA 0.10 mM Mg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mM β-NADH 0.8 mM F-6-P 12 mM Bovine Serum Albumin 5 mg/mL Sugar Stabilizer 5 mg/mL PPi-PFK 0.5 units/mL Aldolase 7.5 units/mL GDH 5 units/mL TPI 50 units/mL |
| Quality【质量】 | A coupled enzyme system containing fructose-6-phosphate kinase (pyrophosphate-dependent), aldolase, triosephosphate isomerase, and α-glycerophosphate dehydrogenase. |
| Principle【原理】 | Two moles of NADH are oxidized to NAD+ per mole of pyrophosphate consumed. The reaction is monitored spectrophotometrically at 340 nm. The reagent can be used to detect 10-100 nmoles of pyrophosphate. |
| Preparation Note【制备说明】 | Dissolve the contents of one vial of Pyrophosphate Reagent in 4.0 mL of water. |
产品性质
| Quality Level【质量水平】 | 200 |
| form【形式】 | powder |
| usage【用途】 | 1 vial sufficient for ≤4 assays |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | A technical bulletin is included. |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |
