Neuropoietin murine

NA.32

Neuropoietin is a newly identified member of the IL-6 cytokine family. Members of this family, including IL-6, IL-11, oncostatin M, leukemia inhibitory factor (LIF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine, and CNTF, display a four-helix bundle structure, and signal through gp130-containing receptor complexes. Neuropoietin, which is predominantly expressed in neuroepithelia during embryonic life, acts through a receptor complex comprising CNTF receptor-α component, gp130, and LIF receptor. Like CNTF, it promotes the survival of embryonic motor neurons and could increase the proliferation of neural precursor cells in the presence of EGF and FGF-2. Interestingly, the human Neuropoietin gene has evolved toward a pseudogene, suggesting that the alternative signaling via CNTF is an effective compensatory pathway. Recombinant murine Neuropoietin is a 19.8 kDa protein containing 183 amino acid residues.

Sterile filtered through a 0.2 micron filter. Lyophilized from 2.5 mM Tris, pH 10.2 and 0.5 mM DTT.

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃.

mouse

expressed in E. coli

≥98% (SDS-PAGE)

lyophilized powder

19.8 kDa

pkg of 10 and 50 μg

<1 EU/μg endotoxin (LAL test)

P83714

wet ice

−20℃

mouse ... Ctf2(244218)

GHS07

Warning

H319

P264 - P280 - P305 + P351 + P338 - P337 + P313

Eye Irrit. 2

11 - Combustible Solids

WGK 3