单克隆抗-FLAG^® M2 小鼠抗

NA.32

Monoclonal ANTI-FLAG^® M2-Alkaline Phosphatase is a purified IgG 1 mouse antibody covalently conjugated to calf intestinal alkaline phosphatase (AP). The antibody conjugate binds to FLAG^® fusion proteins and will recognize the FLAG^® epitope at any position in the fusion protein (N-terminal, Met-N-terminal, C-terminal or internal FLAG^® peptides).

Monoclonal ANTI-FLAG^® M2-Alkaline Phosphatase antibody produced in mouse has been used:

• in direct tissue blot immunoassay of sweet orange petioles samples
• in screening internalization of delta opioid receptor
• for screening cell-free protein expression using ELISA

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Solution in Tris buffered saline containing 50% glycerol plus stabilizer and preservative

ANTI-FLAG is a registered trademark of sigma-aldrich Co. LLC
FLAG is a registered trademark of sigma-aldrich Co. LLC

200

mouse

alkaline phosphatase conjugate

purified immunoglobulin

primary antibodies

M2, monoclonal

buffered aqueous glycerol solution

all

~1 mg/mL

indirect ELISA: 1:20,000

IgG1

DYKDDDDK

wet ice

−20℃

12 - Non Combustible Liquids

WGK 3