脂肪氧化酶 来源于大豆

1.13.11.12 ( BRENDA | IUBMB )

232-853-1

MFCD00131527

NA.54

Lipoxidases (LOXs) are nonheme iron proteins. It is widely present in plants, some microorganisms, and animals. They are categorized as LOX-1, LOX-2, and LOX-3 considering pH and optimum temperature, substrate specificity, and level of inhibition by different agents.

Lipoxidase from Glycine max (soybean) has been used:

• to study the effects of 2-(α-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol(TMG) on oxidation of lipoproteins
• as a representative enzyme for lipoxygenases in microplate assay platform to screen for enzymes degrading wood extractives
• as a positive control in lipoxygenase inhibitory activity assay

大豆酶将使用花生四烯酸作为底物，以亚油酸作为底物的活性约为 15%；花生四烯酸氧化的产物是 12-或 15-过氧化氢花生四烯酸(12-HPETE 或 15-HPETE)。

Lipoxidases are deactivated by heat treatment near 100℃ for 5 to 10 minutes. Studies show that gamma irradiation treatment decreases the specific activity of soybean lipoxygenases. These enzymes are used in studies related to asthma and allergies. Lipoxidases are applicable for industrial bio-transformations such as in the production of aroma fine chemicals.
催化含有顺式，顺式-1,4-戊二烯结构的脂质的氢过氧化。

当亚油酸为 3.0 mL 体积(1 cm 光径)底物时，25℃、pH 9.0 条件下 1 个单位每分钟将导致 A ₂₃₄ 增加 0.001。一个 A ₂₃₄ 单位相当于氧化 0.12μ 摩尔亚油酸。

含有稳定剂和 NaCl

200

Type I-B

lyophilized powder

≥50,000 units/mg solid

108 kDa (two 54 kDa subunits)

wet ice

−20℃

15000000, 75000000, 150000000 units in poly bottle

GHS08

Danger

H334

P261 - P284 - P501

Resp. Sens. 1

11 - Combustible Solids

WGK 1

Eyeshields, Gloves, type N95 (US)