来自西非单叶豆(加纳籽)的凝集素

NA.32

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

BS-I对末端α-D-半乳糖残基具有主要亲和力，对末端N-乙酰基-α-D-半乳糖胺残基具有二级亲和力。

BS-I是一种四聚体凝集素，由两种类型的亚基A和B组成。存在由不同亚基组成的五种BS-I同工凝集素：BSI-B₄、BSI-AB₃、BSI-A₂B₂、BSI-A₃B、以及BSI-A₄。BSI-B₄ 是血型B特异的，其对末端α-D-半乳糖残基具有独特的亲和力，而BSI-A₄ 具有血型A特异性，其对末端N-乙酰基-α-D-半乳糖胺残基具有主要亲和力。

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 ℃.

Contains sodium citrate buffer salts and calcium chloride

Prepared from peroxidase type VI using a modification of the method of O′Sullivan, et al. Repurified by affinity chromatography after conjugation.

凝集活性以 μg/mL表示，并且使用磷酸盐缓冲盐水(pH6.8)对1mg/mL溶液进行连续稀释液来测定，对于每种凝集素，所含有钙、镁和锰的浓度不同。该活性是在25℃孵育1小时后，对2％合适的红细胞悬浮液进行凝集所需的最低浓度。

200

peroxidase conjugate

≥90% protein basis (Warburg-Christian)

lyophilized powder

<200 μg per mL agglutination activity (using human blood group B erythrocytes)

40-160 units/mg protein

Protein, ~95% modified Warburg-Christian

blood typing: suitable
fractionation: suitable

−20℃

13 - Non Combustible Solids

WGK 3

Eyeshields, Gloves, type N95 (US)