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Lambda DNA EcoR I Hind III Digest
基本信息
| NACRES | NA.25 |
| General description【一般描述】 | Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. |
| Application【应用】 | Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels. Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products. |
| Components【组分】 | 10mM Tris-HCl 中的溶液，pH 8.0，1mM EDTA |
| Other Notes【其他说明】 | Contains 13 fragments, 125–21,226 bp For optimal resolution, the recommended agarose gel concentration is 0.7%. |
| Preparation Note【制备说明】 | Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction. |
产品性质
| Quality Level【质量水平】 | 200 100 |
| form【形式】 | liquid |
| usage【用途】 | 100 uses (0.1 mg size) 500 uses (0.5 mg size) |
| suitability【适用性】 | suitable for electrophoresis (DNA) |
| storage temp.【储存温度】 | −20℃ |
安全信息
| Pictograms【象形图】 | GHS07 |
| Signal word【警示用语：】 | Warning |
| Hazard Statements | H317 |
| Precautionary Statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| Hazard Classifications【危险分类】 | Skin Sens. 1 |
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 1 |
